a303 998a t rrid ab 2620347 ccdc111 primpol proteintech (Proteintech)
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a303 998a t rrid ab 2620347 ccdc111 primpol proteintech
A303 998a T Rrid Ab 2620347 Ccdc111 Primpol Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ccdc111+primpol/Hsc70+Antibody/pmc12188298__gkaf573_supplemental_file-4-179-183
Average 96 stars, based on 167 article reviews
A303 998a T Rrid Ab 2620347 Ccdc111 Primpol Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ccdc111+primpol/Hsc70+Antibody/pmc12188298__gkaf573_supplemental_file-4-179-183
Average 96 stars, based on 167 article reviews
a303 998a t rrid ab 2620347 ccdc111 primpol proteintech - by Bioz Stars,
2026-09
96/100 stars
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Centrifugation:Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis Article Snippet: To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), CtIP (1:500, Santa Cruz, sc-271339), BPTF (1:500, Millipore Sigma, ABE24), PCNA (1:200, Santa Cruz, sc-56), MRE11 (1:500, Cell Signaling Tech, 4895S), DNA2 (1:500, Thermo Fisher Scientific, PA5-77943), SMARCAL1 (1:500, Santa Cruz, sc-376377), ZRANB3 (1:500, Bethyl, A303-033A), HLTF (1:1000, Abcam, ab183042), Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis. Article Snippet: Whole cell lysate extraction and immunoblotting To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), CtIP (1:500, Santa Cruz, sc-271339), BPTF (1:500, Millipore Sigma, ABE24), PCNA (1:200, Santa Cruz, sc-56), MRE11 (1:500, Cell Signaling Tech, 4895S), DNA2 (1:500, Thermo Fisher Scientific, PA5-77943), SMARCAL1 (1:500, Santa Cruz, sc-376377), ZRANB3 (1:500, Bethyl, A303-033A), HLTF (1:1000, Abcam, ab183042), Isolation:Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis Article Snippet: To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), CtIP (1:500, Santa Cruz, sc-271339), BPTF (1:500, Millipore Sigma, ABE24), PCNA (1:200, Santa Cruz, sc-56), MRE11 (1:500, Cell Signaling Tech, 4895S), DNA2 (1:500, Thermo Fisher Scientific, PA5-77943), SMARCAL1 (1:500, Santa Cruz, sc-376377), ZRANB3 (1:500, Bethyl, A303-033A), HLTF (1:1000, Abcam, ab183042), Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis. Article Snippet: Whole cell lysate extraction and immunoblotting To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), CtIP (1:500, Santa Cruz, sc-271339), BPTF (1:500, Millipore Sigma, ABE24), PCNA (1:200, Santa Cruz, sc-56), MRE11 (1:500, Cell Signaling Tech, 4895S), DNA2 (1:500, Thermo Fisher Scientific, PA5-77943), SMARCAL1 (1:500, Santa Cruz, sc-376377), ZRANB3 (1:500, Bethyl, A303-033A), HLTF (1:1000, Abcam, ab183042), Western Blot:Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis Article Snippet: To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), CtIP (1:500, Santa Cruz, sc-271339), BPTF (1:500, Millipore Sigma, ABE24), PCNA (1:200, Santa Cruz, sc-56), MRE11 (1:500, Cell Signaling Tech, 4895S), DNA2 (1:500, Thermo Fisher Scientific, PA5-77943), SMARCAL1 (1:500, Santa Cruz, sc-376377), ZRANB3 (1:500, Bethyl, A303-033A), HLTF (1:1000, Abcam, ab183042), Article Title: SNF2L suppresses nascent DNA gap formation to promote DNA synthesis. Article Snippet: Whole cell lysate extraction and immunoblotting To obtain whole-cell lysates, cell pellets were incubated on ice for 30 min in lysis buffer (50 mM Tris–Cl pH = 7.4, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM aprotinin, 1 mM leupeptin, 1 mM DTT, 1 mM PMSF) supplemented with Pierce universal nuclease and 1 mM MgCl 2 . .. After centrifugation for 30 min, supernatant was isolated and quantified prior to immunoblotting with the following antibodies: SMARCA1 (1:1000, Cell Signaling Tech, 9450), SMARCA5 (1:500, Novus Bio, NB100-55301), RECQ1 (1:500, Abcam, ab172607), MUS81 (1:500, Santa Cruz, sc-53382), CtIP (1:500, Santa Cruz, sc-271339), BPTF (1:500, Millipore Sigma, ABE24), PCNA (1:200, Santa Cruz, sc-56), MRE11 (1:500, Cell Signaling Tech, 4895S), DNA2 (1:500, Thermo Fisher Scientific, PA5-77943), SMARCAL1 (1:500, Santa Cruz, sc-376377), ZRANB3 (1:500, Bethyl, A303-033A), HLTF (1:1000, Abcam, ab183042), |